Plasmid_Backbone

Part:BBa_K106698:Design

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-29)


AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal NotI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal BamHI site found at 4031
    Illegal XhoI site found at 3965
    Illegal XhoI site found at 4950
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal NgoMIV site found at 3133
    Illegal NgoMIV site found at 4561
    Illegal AgeI site found at 5857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1430
    Illegal SapI.rc site found at 408
    Illegal SapI.rc site found at 2512


Design Notes

LexA Ops were cloned non-directionally into the PspOMI site. Inner site is broken, thus the cassette can be subcloned from this plasmid by PspOMI/SacI digestion (for example, to change markers).


Source

Modification of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).

References